Protein Disulfide Isomerase (PDI) (Human) ELISA Kit

Alternate Name :

Tag Line :

A Sandwich ELISA kit for the quantitative measurement of human PDI in human serum, plasma, culture supernatants and other biological fluids.

Storage Conditions: 4°C

Shelf life: 6 months

Summary :

• Detection method- Absorbance (450 nm)
• Species reactivity- Human
• Application- quantitative measurement of PDI in human serum, plasma, tissue homogenates and other biological fluids.

Detection Method: Absorbance (450 nm)

Features & Benefits:

Easy, convenient and time-saving method to measure the level of PDI in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Detection Range: 0.313-20 ng/ml
Sensitivity: < 0.188 ng/ml
Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%

Description:

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. BioVision’s PDI ELISA kit is a sandwich ELISA assay for the quantitative measurement of human PDI in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human PDI captured from the samples.