Protein Carbonyl Content Assay Kit
Kits
- 貨號 Cat. No.
- K830-100
- 規格 Size
- K830-100
- Sample Type
- Variable (protein containing samples)
Description
Alternate Name :
Tag Line :
Simple assay to quantify protein carbonyls
Storage Conditions: +4°C
Shelf life: 12 months
Summary :
• Detection method- Absorbance ( 375 and 562 nm)
• Species reactivity- Mammalian
Detection Method:
Features & Benefits:
• Simple procedure; takes ~ 30-90 minutes
Description:
Protein carbonyl groups are an important and immediate biomarker of oxidative stress. DNPH tagging of protein carbonyls has been one of the most common measures of oxidative stress. DNP hydrazones formed from the reaction are easily quantifiable at 375 nm. BioVision’s Protein Carbonyl Content Assay Kit is designed to provide a simple and accurate method of quantifying carbonyls in protein samples. Using BSA as an example, a 1 mg (~ 15 nmol) sample has a detection limit of about 0.15 nmol carbonyl, where BSA typically contains approximately 1-3 nmol carbonyl/ mg.