Prosaposin (PSAP), DIY ELISA Kit

貨號 Cat. No.: MBS2089303-5x96Tests
規格 Size: 5x96 Tests
品牌 Brand: MyBioSource

Description

Product Name	Prosaposin (PSAP), DIY ELISA Kit â˜…Popular Itemâ˜…
Also Known As	Prosaposin (PSAP) ELISA Kit DIY Materials
Product Synonym Names	SAP1; GLBA; CSAct; Dispersin; Pro-Saposin; Proactivator polypeptide; Glucosylceramidase activator; Sulfatide/GM1 activator; Cerebroside sulfate activator; Sphingolipid Activator 1
Product Gene Name	PSAP diy elisa kit	[Similar Products]
Research Use Only	For Research Use Only. Not for use in diagnostic procedures.
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Sample Manual Insert
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MBS2089303 COA

OMIM	J03077 mRNA

Species Reactivity	Human

Specificity	The Abs in the kit have high sensitivity and excellent specificity for detection of Prosaposin (PSAP). No significant cross-reactivity or interference between Prosaposin (PSAP) and analogues was observed.

Assay Type	Double-antibody Sandwich ELISA for Antigen Detection
Samples	Serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, seminal plasma, cell culture supernates and other biological fluids.
Detection Range	0.156-10ng/mL
Sensitivity	0.067ng/mL
Reagent Contents	Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage	1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours. 2. Aspirate and wash 1 time. 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours. 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required	ELISA/CLIA Support Pack 1. (Catalog MBS2089471)

Preparation and Storage	Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Note	Our DIY ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes	Small volumes of PSAP diy elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.