Progranulin (mouse) ELISA Kit

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Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Absorbance (450 nm)
• Species reactivity- Mouse

Detection Method:

Features & Benefits:

The lowest level of progranulin that can be detected by this assay is 60 pg/ml.
The Assay range is 0.125 ng/ml – 8 ng/ml.
This ELISA is specific for the measurement of natural and recombinant mouse progranulin. It does not cross-react with mouse RBP4, mouse adiponectin, mouse Nampt, mouse leptin, mouse RELM- β, mouse IL-33, mouse IDO, mouse resistin, mouse clusterin, mouse ANGPTL3, mouse ANGPTL4, human vaspin, human adiponectin, human progranulin, rat Nampt.

Description:

BioVision’s Progranulin (mouse) ELISA kit is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of progranulin in the samples.