Pro-SUMO-2
- 貨號 Cat. No.
- UL-750
- 規格 Size
- 500 μg
- 品牌 Brand
- Boston Biochem
Description
Pro-SUMO-2
human recombinant
Product Information
500 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual protein concentration will vary with specific Lot #.
10.9 kDa
> 95% by SDS-PAGE
Use & Storage
Typical in vitro concentrations are 10-50 μM depending on conditions.
SUMO1-agarose can be re-used for at least 10-15 applications if properly maintained. After use, clean resin by washing with 10 ml 50 mM Tris-Cl pH9.0, 1 M KCl. Remove cleaning solution by equilibrating resin in 25 mM HEPES (or similar buffer) pH 7.5, containing either 1 mM NaN3 or 10-20 % ethanol. Resin should be stored at 4°C. DO NOT FREEZE
Catalog #: UL-750
Pro-SUMO-2
Product Source:
human recombinant
Also known as: Pro-SUMO-2 (SMT3A)
All SUMO isoforms are translated with additional C-terminal residues that have to be removed to generate the active protein. Pro-SUMO-2 (95 amino acids) is the inactive precursor of SUMO-2 (93amino acids) and is processed at the C-terminus by SUMO-2 specific proteases (SENPs). The resulting SUMO-2 protein has the conserved C-terminal Gly-Gly residues that function in activation and conjugation reactions. This protein can be used as a negative control in sumoylation reactions or as a substrate for SENPs. NCBI Accession # NM_006937.
Product Information
Quantity:
500 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual protein concentration will vary with specific Lot #.
MW:
10.9 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical in vitro concentrations are 10-50 μM depending on conditions.
Storage:
SUMO1-agarose can be re-used for at least 10-15 applications if properly maintained. After use, clean resin by washing with 10 ml 50 mM Tris-Cl pH9.0, 1 M KCl. Remove cleaning solution by equilibrating resin in 25 mM HEPES (or similar buffer) pH 7.5, containing either 1 mM NaN3 or 10-20 % ethanol. Resin should be stored at 4°C. DO NOT FREEZE