PRMT5 Antibody (LS-B2433)

貨號 Cat. No.: LS-B2433
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2433
Antibody Validation:	This antibody replaces catalog number LS-C16093. It has been validated for use in the following assays: IHC-P.
Antibody Target:	PRMT5
Synonyms:	PRMT5 Antibody, HRMT1L5 Antibody, IBP72 Antibody, JBP1 Antibody, prmt5 Antibody, skb1 Antibody, SKB1Hs Antibody
Host:	PRMT5 antibody was produced in Rabbit
Antigen Species:	PRMT5 antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Synthetic peptide
Immunogen:	PRMT5 antibody was raised against synthetic peptide (KLH coupled) corresponding to residues surrounding amino acid 100 of human Skb1Hs methyltransferase.
Antibody Specificity:	Recognizes endogenous levels of total human Skb1Hs methyltransferase protein at 70kD. Does not cross-react with other related mammalian methyltransferases. Species cross-reactivity: monkey, mouse and rat.
Antigen Modification:	aa100
Predicted Species Reactivity:	Monkey, Mouse, Rat
Purification:	Protein A Column
Presentation:	10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2433 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2433 was determined to be 1:100.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (1:100), Western blot (WB) (1:1000), Immunoprecipitation (IP) (1:100) (Optimal dilution to be determined by the researcher)
Size:	50 µl
Concentration:	0.2 mg/ml