Presepsin (Human) ELISA Kit

Alternate Name : sCD14 ELISA Kit,soluble CD14 subtype ELISA Kit,Presepsin ELISA Kit

Tag Line :

A Sandwich ELISA kit for the qualitative detect Presepsin in human serum or plasma.

Storage Conditions: 4°C

Shelf life: 6 months

Summary :

• Detection method- Absorbance (450 nm)
• Species reactivity- human
• Application- quantitative measurement of Presepsin in serum, plasma, tissue homogenates and other biological fluids.

Detection Method: Colormetric

Features & Benefits:

Easy, convenient and time-saving method to measure the level of Presepsin in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.,No significant cross-reactivity or interference was observed,Sensitivity: < 0.094 ng/ml,Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%,Detection Range: 0.156-10 ng/ml

Description:

Presepsin is a soluble subtype CD14. It is considered a novel biomarker for sepsis diagnostic and prognosis evaluation. BioVision’s Presepsin ELISA kit is a sandwich ELISA assay for the quantitative measurement of Presepsin in human serum, plasma and cell culture supernatants. Anti-Presepsinantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Presepsin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Presepsin amount of sample captured in plate.