Pregnanediol-3-Glucuronide (PDG) ELISA Kit

Alternate Name :

Tag Line :

A competitive ELISA kit for the quantitative measurement of PDG in dried fecal, urine and tissue culture samples.

Storage Conditions: 4ºC

Shelf life: 12 months

Summary :

Detection Method: Absorbance (450 nm)

Features & Benefits:

This ELISA kit is used for in vitro quantitative determination of PDG.,Sensitivity: 0.180 ng/mL,Detection Limit: 0.320 ng/mL,Detection Range: 0.391 - 50 ng/mL

Description:

Pregnanediol Glucuronide, C27H44O8, also known as PDG (5β-Pregnan-3a, 20a-diol 3-glucosiduronate) is the major metabolite of progesterone. Progesterone is an essential regulator of human female reproductive function in the uterus, ovary, mammary gland and brain, and plays an important role in non-reproductive tissues such as the cardiovascular system, bone and the central nervous system. BioVision’s PDG ELISA kit is a competitive ELISA assay for the quantitative measurement of PDG in urine and fecal samples. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A PDG-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to PDG to each well. After incubation the plate is washed and substrate is added. The substrate reacts with the bound PDG-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength.