Proteins and Peptides
PPP3R1 Antibody (LS-B2813)
- 貨號 Cat. No.
- LS-B2813
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2813 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C21068. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | PPP3R1 |
| Synonyms: | PPP3R1 Antibody, calcineurin b subunit isoform 1 Antibody, CALNB1 Antibody, cna2 Antibody, CNB Antibody, CNB1 Antibody, ppp3r1 Antibody, protein phosphatase 2b regulatory subunit Antibody |
| Family: | Protein Phosphatase Regulatory Subunit |
| Subfamily: | not assigned-Protein Phosphatase Non Catalytic |
| Host: | PPP3R1 antibody was produced in Rabbit |
| Antigen Species: | PPP3R1 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Recombinant protein |
| Immunogen: | PPP3R1 antibody was raised against recombinant human Calcineurin/PP2B B expressed in E. coli containing substitutions of Ala for Cys11 and Lys for Cys153. |
| Antibody Specificity: | Recognizes human Calcineurin B, Mr 19 kD. |
| Predicted Species Reactivity: | Bovine, Drosophila, Mouse, Rat, Yeast |
| Purification: | Ammonium Sulfate Precipitation |
| Presentation: | 0.1M Tris-glycine, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2813 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2813 was determined to be 5 ug/ml. Immunoprecipitation: 4 ug immunoprecipitates Calcineurin B from 500 ug of rat brain cytosol preparation. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (0.5 - 2 µg/ml), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
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