PPARGC1 Antibody (LS-B2569)

Description

Antibody Catalog ID:	LS-B2569
Antibody Validation:	This antibody replaces catalog number LS-C17262. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Peroxisome Proliferator-activated Receptor Gamma, Coactivator 1 (PPARGC1)
Host:	Rabbit
Antigen Species:	Human
Clonality:	Polyclonal
Antigen Type:	Synthetic peptide
Immunogen:	Synthetic peptide corresponding to aa 75-90 NIFEKIDEENEANLLA of human PGC-1a.
Antibody Specificity:	Recognizes human PGC-1a and PGC-1b. Species cross-reactivity: mouse and rat.
Antigen Modification:	aa75-90
Predicted Species Reactivity:	Mouse, Rat
Purification:	Affinity Chromatography
Presentation:	TBS, pH 7.4, 0.5 mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Recommended Storage:	Long term: -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2569 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2569 was determined to be 5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1:200), ELISA (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml