Proteins and Peptides
PLN Antibody (LS-B2358)
- 貨號 Cat. No.
- LS-B2358
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2358 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C26050. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Phospholamban (PLN) |
| Synonyms: | PLN |
| Host: | PLN antibody was produced in Mouse |
| Antigen Species: | PLN antibody was raised against Dog |
| Clonality: | Monoclonal |
| Isotype: | IgG2a |
| Antigen Type: | Synthetic peptide |
| Immunogen: | PLN antibody was raised against synthetic peptide corresponding to aa 2-25 (D2KVQYLTRSAIRRASTIEMPQQAR25) of dog PLB (phospholamban). |
| Antibody Specificity: | Recognizes canine PLB. Recognizes an epitope between aa 9-17 of canine PLB. Detects bands of ~25kD (non-reducing conditions; representing the pentameric form of PLB) and ~5kD (reducing conditions; representing the monomeric form of PLB) by Western Blot. Species cross-reactivity: human, mouse, rat, rabbit chicken, sheep, porcine and guinea pig. |
| Antigen Modification: | aa2-25 |
| Predicted Species Reactivity: | Dog, Guinea pig, Human, Mouse, Rat, Sheep |
| Purification: | Protein A Column |
| Presentation: | PBS, 0.05% sodium azide. |
| Recommended Storage: | Long term: Add glycerol (40-50%) -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2358 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2358 was determined to be 5 ug/ml. Western Blot: 2 ug/ml Detects bands of ~25kD (non-reducing conditions; representing the pentameric form of PLB) and ~5kD (reducing conditions; representing the monomeric form of PLB). Function: Increases the calcium uptake in ventricular SR vesicles. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunocytochemistry (ICC), Western blot (WB), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
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