PLC/PRF/5 (Alexander)
- 貨號 Cat. No.
- CLC031
- 品牌 Brand
- gene-rl
Description
.jpg)
Cell Lines (GRL-CLC031) | |
Designations | PLC/PRF/5 (Alexander) |
| Cell type | Human hepatocellular carcinoma |
Growth property | Adherent |
Seeding density(cells/ml) | 2.0x106 |
survival rate | 89% |
Culture medium | 90%MEM (Eagle) in Earle’s BSS with 0.1mM non-essential amino acids 10%FBS |
| Incubation condition | 370C 5% CO2 |
Freezed medium | 95% culture medium+5%DMSO |
Medium renewal | every 2 to 3 days |
| Subculturing |
Remove medium, and rinse with PBS solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
|
Mycoplasma test | Negative for bacteria，fungi and mycoplasma. |
Advertence: |
When you receive the cell line, please examine the flask and its cap. If it is not well-found, please tell us instantly. After examination, please wipe the flask and flask cap with 70% alcohol, and then loosen it. Afterward, put the flask into incubator immediately.