PKM2 Antibody (LS-B2445)

貨號 Cat. No.: LS-B2445
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2445
Antibody Validation:	This antibody replaces catalog number LS-C38682. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Pyruvate Kinase, Muscle (M1 and M2 isoforms) (PKM2)
Synonyms:	PKM2, pyruvate kinase, m1
Host:	PKM2 antibody was produced in Rabbit
Antigen Species:	PKM2 antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Synthetic peptide
Immunogen:	PKM2 antibody was raised against synthetic peptide derived from the sequence of human PKM2 (KLH). Molecular Weight: 60kD.
Antibody Specificity:	Recognizes endogenous levels of total human PKM2 protein at 60kD. Species cross-reactivity: monkey, mouse and rat.
Predicted Species Reactivity:	Monkey, Mouse, Rat
Purification:	Immunoaffinity Chromatography
Presentation:	10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2445 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2445 was determined to be 1:100.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (1:100), Immunohistochemistry - Frozen (IHC-Fr) (1:100), Western blot (WB) (1:1000) (Optimal dilution to be determined by the researcher)
Size:	50 µl