PKG Antibody

Antibody Target :PKG

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic peptide near the N-terminus of human PKG

Accession#: ABQ59040

Gene ID: 5592

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms/rt/ck/ht/bv/rb

Application: WB/IP/IHC

Application & Usage: The antibody can be used for Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes 75 and 85 kDa PKG, corresponding to the molecular mass of two PKG isoforms.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: PKGs are cyclic GMP-dependent protein kinases (also designed cGKs) that are classified into two types, PKGI and PKGII. Studies have shown that cGKs and cyclic AMP-dependent kinases (cAKs) are highly homologous protein kinase families with similar substrate specificities. Phosphorylation of cellular proteins by both families of kinases leads to alterations in calcium mobilization, protein phosphatase activity, ion channel function, gene transcription, smooth muscle contractility and platelet aggregation. However, recent studies using mice deficient in PKGs have shown that cGMP kinases regulate very specifically distinct pathways which are separate from those used by cyclic AMP kinases.