PicoProbeTM Methylglyoxal Assay Kit (Fluorometric)
Kits
- 貨號 Cat. No.
- K461-100
- 規格 Size
- K461-100
- Reactivity
- Eukaryotes
- Sample Type
- • Tissue Homogenates and Cell Lysates: Mouse Liver 、 etc. • Biological Fluids: Cerebrospinal Fluid (CSF) 、 etc.) 、 Jurkat cells 、 Rat Brain
Description
Alternate Name :
Tag Line :
First fluorometric kit in the market that can detect Methylglyoxal in samples
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Fluorescence (RFU: 535/587 nm)
• Species reactivity- Eukaryotes
• Applications- This Kit provides a simple, highly sensitive in vitro assay for detecting Methylglyoxal levels in biological samples.
Detection Method: Fluorescence (Ex/Em 535/587nm)
Features & Benefits:
This Kit provides a simple, highly sensitive in vitro assay for detecting Methylglyoxal levels in biological samples.
Description:
Methylglyoxal ( mg, 2-oxopropanal, pyruvic aldehyde), a by-product of glycolysis, is a highly toxic, reactive dicarbonyl compound. It causes non-enzymatic glycation of proteins which yields irreversible advanced glycation end products (AGEs), leading to cross-linking or degradation of proteins. mg is associated with several pathologies including diabetes, aging and neurodegenerative diseases. Furthermore, recent studies have suggested that mg may induce carbonyl stress-type schizophrenia. BioVision’s PicoProbeTM Methylglyoxal Assay Kit (Fluorometric) can detect mg using an enzyme-coupled reaction which reduces the fluorogenic probe. The reduced fluorophore produces a stable signal (Ex/Em= 535/587nm), which is directly proportional to the amount of mg in samples. The assay is simple, reproducible and can specifically detect as low as 6 pmol of mg in a 100 µl reaction.