PicoProbe™ Glucose-6-Phosphate Fluorometric Assay Kit

Alternate Name :

Tag Line :

Highly Sensitive Assay

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Fluorescence (Ex/Em 535/587 nm)
• Species reactivity- Mammalian
• Applications- The kit can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 µM in the original sample, in a high-throughput screening (HTS) format.

Detection Method:

Features & Benefits:

• Simple procedure; takes ~ 35-40 minutes to complete the assay
• Fast and convenient
• Sensitive, efficient and accurate assay

Description:

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells which then enters either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. BioVision's glucose-6-phosphate Assay Kit is a simple, sensitive and rapid means of quantifying G6P in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product that converts a nearly colorless probe to an intensely fluorescent product (Ex/Em 535/587 nm). The Glucose-6-phosphate Assay Kit can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 µM in the original sample assuming a dilution of 5X during processing. The PicoProbe™ G6P Assay Kit is much more sensitive than the G6P Colorimetric Assay Kit for low G6P samples.