PicoProbe™ Fructose-6-Phosphate Fluorometric Assay Kit
Kits
- 貨號 Cat. No.
- K689-100
- 規格 Size
- K689-100
- Sample Type
- as well as many other biological fluids 、 Cell and tissue culture supernatants 、 growth media and food products 、 Plasma and Serum 、 urine
Description
Alternate Name :
Tag Line :
Highly Sensitive Assay
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Fluorescence (Ex/Em 535/587 nm)
• Species reactivity- Mammalian
• Applications- The PicoProbe™ F6P Assay Kit can detect F6P in the range of 0.01 to 0.5 nmoles with detection sensitivity ~1 µM of F6P.
Detection Method:
Features & Benefits:
• Simple procedure; takes ~ 35-40 minutes to complete the assay
• Fast and convenient
• Sensitive, efficient and accurate assay
Description:
Fructose-6-phosphate (F6P) is an important intermediate in the glycolytic pathway which leads from glucose to pyruvate. It is formed from glucose-6-phosphate and is further phosphorylated to fructose-1,6-diphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. The transformation of glucose-6-phosphate is controlled by phosphoglucose isomerase, a very interesting enzyme in that it possesses multiple functions, as an isomerase, a neuroleukin, an autocrine motility factor and a differentiation and maturation mediator (1). BioVision's PicoProbe ™ Fructose-6-phosphate Assay Kit is a fluorescence-based simple, highly sensitive and rapid means of quantifying F6P in a variety of samples. In the assay, F6P is converted to glucose-6-phosphate which is subsequently oxidized with the generation of a fluorescent product. The PicoProbe™ F6P Assay Kit can detect F6P in the range of 0.01 to 0.5 nmoles with detection sensitivity ~1 µM of F6P.