PhosphoSeek™PDE5A Assay Kit

 

400 assays. A sensitive and high speed homogeneous assay for detection of enzyme activity and for screening of Phosphodiesterase 5A (PDE5A) inhibitors in a high-throughput screening (HTS) format. The assay uses a proprietary Sensor, which is a fluorescent molecule containing a trivalent metal ion, that binds to phosphorylated biological substrates. Phosphorylation is measured by the change of fluorescence of a dye-labeled and phosphorylated substrate when bound by the Sensor. The change in fluorescence directly correlates to the level of substrate conversion.