Phospho-TrkA Antibody

Antibody Target :Phospho-TrkA

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic phospho-peptide corresponding to resides surrounding Tyr490 of human TrkA

Accession#: NP_001012331

Gene ID: 4914

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-Phospho-TrkA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.

Purification: Affinity purified

Species Reactivity: hu/ms/rt

Application: WB/IP/IF

Application & Usage: Western blotting (1 µg/ml), immunoprecipitation (4-8 µg/ml), and immunocytochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 140 kDa TrkA only when phosphorylated at Tyr490.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: TrkA, the high affinity Nerve Growth Factor (NGF) receptor, autophosphorylates on tyrosine to activate multiple effectors. Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at these sites reflects Trk kinase activity.