Phospho-Stat1 Polyclonal Ab

HOST: Rabbit

FORMULATION: 100 ug (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.

SPECIES REACTIVITY: human, mouse, rat

STORAGE CONDITIONS: Store at -20oC. For long term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles.

BACKGROUND: Membrane receptor signaling by various ligands, including interferons and growth hormones, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3ß appears to be activated by both while Stat3a is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

SPECIFICITY: The antibody recognizes 91 kDa a-isoform and 84 kDa ß­isoform of phosphorylated Stat1 (Tyr701) of human, mouse, and rat origins. Reactivity to other species has not been tested.

APPLICATION: The antibody can be used in Western blotting (1-3 ug/ml) and immunofluorescence (10-20 ug/ml). However, the optimal concentrations should be determined individually. USAGE:

For Research Use Only. Not to be used in human.