Phospho-Ser/Thr/Tyr Antibody (LS-B2784)

Description

Antibody Catalog ID:	LS-B2784
Antibody Validation:	This antibody replaces catalog number LS-C50592. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Phospho-Ser/Thr/Tyr
Host:	Mouse
Antigen Species:
Clonality:	Monoclonal
Isotype:	IgG1
Antibody Clone Name:	SPM101
Immunogen:	Phosphoserine, phosphothreonine, and phosphotyrosine conjugated to protein carriers
Predicted Species Reactivity:	All
Purification:	Protein G Column
Presentation:	10 mM PBS, pH 7.4, BSA, sodium azide.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2784 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2784 was determined to be 10 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (1 - 2 µg/ml), Immunoprecipitation (IP) (2 µg/mg lysate), Immunofluorescence (IF) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.2 mg/ml