Phospho-Raf Antibody

Antibody Target :Phospho-Raf (THR259)

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic phospho-peptide surrounding amino acid Ser259 of human c-Raf

Accession#: P04049

Gene ID: 5894

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-Phospho-Raf (Ser259) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms/rt

Application: WB/IHC

Application & Usage: Western blotting (0.5-4 µg/ml) and immunohistochemistry (20-40µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~74 kDa c-Raf when phosphorylated at Ser259.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: c-Raf is a mitogen-activated protein kinase. Binding of Raf to 14-3-3 is required for c-Raf kinase activity, and also blocks the ability of phosphatase to inhibit c-Raf. 14-3-3 binds c-Raf at two phospho-serine residues: Ser259 and Ser621. Mutation of Ser259 to alanine constitutively activates Raf kinase activity, but mutation of Ser621 to alanine results in an inactive kinase.