Phospho-PKC Antibody

Antibody Target :Phospho-PKC

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic phospho-peptide surrounding amino acid Ser660 of human PKC beta II

Accession#: NP_002729

Gene ID: 5579

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.

Purification: Affinity purified

Species Reactivity: hu/ms/rt

Application: WB

Application & Usage: The antibody can be used in Western blotting (1-2 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes several Phosphorylated PKC (Ser660) isoforms of human, mouse, and rat origins. Reactivity to other species has not been tested.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: PKC (Protein kinase C) is a calcium-dependent and phospholipid-dependent enzyme that is activated in vivo by the lipid diacylglycerol. PKC has been shown to be involved in signal transduction and various aspects of neoplastic transformation. PKC contains an amino terminal regulatory domain and a carboxy terminal catalytic domain joined by a hinge region. Cleavage of protein kinase C in vitro results in the generation of two functional fragments corresponding to these two domains.