Phospho-p38 MAPK Antibody

Antibody Target :Phospho-p-38 MAPK

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic phospho-peptide surrounding amino acid Thr180/Tyr182 of human p38 MAP kinase

Accession#: ACI00233

Gene ID: 1432

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 ?g (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms/rt

Application: WB/IP/IHC

Application & Usage: Western blotting (0.5-4 ?g/ml), immunoprecipitation (10-20 mg/ml) and immunohistochemistry (10-20 ?g/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 43 kDa phosphorylated p38 (Thr180/Tyr182) MAP kinase of human, mouse, and rat origins. Reactivity to other species has not been tested.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: p38 MAP kinase is the mammalian homologue of the yeast HOG kinase and participates in a cascade controlling cellular responses to cytokines and stress. Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including inflammatory cytokines, UV light and growth factors, etc. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase-2 and to phosphorylate the transcription factors ATF-2 and Max.