Phospho-FoxO4/AFX Antibody
- 貨號 Cat. No.
- 3270-100
- 規格 Size
- 100 µg
Description
pstrongAntibody Target :/strongPhospho-FoxO4/AFX/p
pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bSynthetic peptide corresponding to residues surrounding Ser193 of human FoxO4/AFX/p
pbAccession#: /bAAI06762.1/p
pbGene ID: /b76827750/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5 mg/ml) peptide affinity purified polyclonal rabbit anti-Phospho-AFX antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide/p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/ms/p
pbApplication: /bWB/p
pbApplication amp; Usage: /bWestern blotting (1-2 micro;g/ml). However, the optimal concentrations should be determined individually. Phospho-AFX (Ser193) antibody detects only the phosphorylated Ser193 of AFX and does not react with nonphosphorylated AFX./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bThe forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias. Within the family, three members (FKHR, AFX, AND FKHRL1) have sequence similarity to the nematode homolog Daf 16, recently shown to mediate signaling via a pathway involving IGFR1, P13-K and Akt. There are three potential Akt phosphorylation sites on the FKHR proteins. One site, FKHR Ser256 is found in the Forkhead domain and the other two are outside this domain. Recent studies show that phosphorylation of FKHR family members by Akt promotes cell survival. Phosphorylation regulates FKHR nuclear translocation and target gene transcription. Insulin also stimulates FKHR phosphorylation at Ser253./p
pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bSynthetic peptide corresponding to residues surrounding Ser193 of human FoxO4/AFX/p
pbAccession#: /bAAI06762.1/p
pbGene ID: /b76827750/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5 mg/ml) peptide affinity purified polyclonal rabbit anti-Phospho-AFX antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide/p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/ms/p
pbApplication: /bWB/p
pbApplication amp; Usage: /bWestern blotting (1-2 micro;g/ml). However, the optimal concentrations should be determined individually. Phospho-AFX (Ser193) antibody detects only the phosphorylated Ser193 of AFX and does not react with nonphosphorylated AFX./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bThe forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias. Within the family, three members (FKHR, AFX, AND FKHRL1) have sequence similarity to the nematode homolog Daf 16, recently shown to mediate signaling via a pathway involving IGFR1, P13-K and Akt. There are three potential Akt phosphorylation sites on the FKHR proteins. One site, FKHR Ser256 is found in the Forkhead domain and the other two are outside this domain. Recent studies show that phosphorylation of FKHR family members by Akt promotes cell survival. Phosphorylation regulates FKHR nuclear translocation and target gene transcription. Insulin also stimulates FKHR phosphorylation at Ser253./p