Phospho-ATF-2 Antibody
- 貨號 Cat. No.
- 3359-100
- 規格 Size
- 100 µg
Description
pstrongAntibody Target :/strongPhospho-ATF-2 (Thr71)/p
pstrongHost: Rabbit /strong/p
pstrongAntibody Type: Polyclonal /strong/p
pstrongIsotype: Rabbit IgG /strong/p
pstrongAntibody Form: Affinity Purified Antibody /strong/p
pstrongImmunogen Sequence: Synthetic phospho-peptide corresponding to residues surrounding Thr71 of human ATF2 /strong/p
pstrongAccession#: P15336 /strong/p
pstrongGene ID: 215274241 /strong/p
pstrongAppearance: Colorless liquid /strong/p
pstrongConcentration: 0.5 mg/ml /strong/p
pstrongFormulation: 100 micro;g (0.5 mg/ml) purified rabbit anti-Phospho-ATF-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal. /strong/p
pstrongPurification: Affinity purified /strong/p
pstrongSpecies Reactivity: hu/ms/rt /strong/p
pstrongApplication: WB/IP/IHC /strong/p
pstrongApplication amp; Usage: Western blotting (1-4 micro;g/ml), immunoprecipitation (10 micro;g/ml), and immunohistochemistry (20 micro;g/ml). However, the optimal conditions should be determined individually. The antibody detects phosphorylated ATF-2. It recognizes both Thr69/Thr71 dually phosphoralated ATF-2 as well as ATF-2 singly phosphorylated ATF-2 in samples of human, mouse, and rat origins. /strong/p
pstrongStorage Temp.: -20 deg;C /strong/p
pstrongShipping : gel pack /strong/p
pstrongShelf Life: 12 months /strong/p
pstrongHandling: The antibody solution should be gently mixed before use. /strong/p
pstrongBrief Background Descriptions: The transcription factor ATF-2 (also called CRE-BP1) is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr69 and Thr71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr69 and Thr71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro./strong/p
pstrongHost: Rabbit /strong/p
pstrongAntibody Type: Polyclonal /strong/p
pstrongIsotype: Rabbit IgG /strong/p
pstrongAntibody Form: Affinity Purified Antibody /strong/p
pstrongImmunogen Sequence: Synthetic phospho-peptide corresponding to residues surrounding Thr71 of human ATF2 /strong/p
pstrongAccession#: P15336 /strong/p
pstrongGene ID: 215274241 /strong/p
pstrongAppearance: Colorless liquid /strong/p
pstrongConcentration: 0.5 mg/ml /strong/p
pstrongFormulation: 100 micro;g (0.5 mg/ml) purified rabbit anti-Phospho-ATF-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal. /strong/p
pstrongPurification: Affinity purified /strong/p
pstrongSpecies Reactivity: hu/ms/rt /strong/p
pstrongApplication: WB/IP/IHC /strong/p
pstrongApplication amp; Usage: Western blotting (1-4 micro;g/ml), immunoprecipitation (10 micro;g/ml), and immunohistochemistry (20 micro;g/ml). However, the optimal conditions should be determined individually. The antibody detects phosphorylated ATF-2. It recognizes both Thr69/Thr71 dually phosphoralated ATF-2 as well as ATF-2 singly phosphorylated ATF-2 in samples of human, mouse, and rat origins. /strong/p
pstrongStorage Temp.: -20 deg;C /strong/p
pstrongShipping : gel pack /strong/p
pstrongShelf Life: 12 months /strong/p
pstrongHandling: The antibody solution should be gently mixed before use. /strong/p
pstrongBrief Background Descriptions: The transcription factor ATF-2 (also called CRE-BP1) is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr69 and Thr71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr69 and Thr71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro./strong/p