Phospho-Akt Antibody
- 貨號 Cat. No.
- 3257-100
- 規格 Size
- 100 µg
Description
pstrongAntibody Target :/strongPhospho-Akt/p
pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bSynthetic phospho-peptide corresponding to residues surrounding Ser473 of mouse Akt/p
pbAccession#: /bP31749/p
pbGene ID: /b60391226/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5 mg/ml) peptide affinity purified rabbit anti-Phopho-Akt (Ser473) polyclonal antibody in phosphate buffered saline (PBS), containing 50% glycerol, 1% BSA, 0.02% thimerosal./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/ms/rt/p
pbApplication: /bWB/IP/p
pbApplication amp; Usage: /bWestern blotting (0.5-4 micro;g/ml) and immunoprecipitation (10-20 micro;g/ml). However, the optimal conditions should be determined individually. The phospho-Akt (Ser473) antibody detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bAkt, also referred to as PKB and RAC, is the cellular homologue of the viral oncogene v-Akt. This protein kinase is activated by insulin and various growth factors and functions in a pathway involving PI3 kinase as activation is sensitive to wortmanin. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis, although the pathways involved have not been identified. Akt is activated by phospholipid binding and phosphorylation within the activation loop at Thr308 and within the C-terminus at Ser473./p
pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bSynthetic phospho-peptide corresponding to residues surrounding Ser473 of mouse Akt/p
pbAccession#: /bP31749/p
pbGene ID: /b60391226/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5 mg/ml) peptide affinity purified rabbit anti-Phopho-Akt (Ser473) polyclonal antibody in phosphate buffered saline (PBS), containing 50% glycerol, 1% BSA, 0.02% thimerosal./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/ms/rt/p
pbApplication: /bWB/IP/p
pbApplication amp; Usage: /bWestern blotting (0.5-4 micro;g/ml) and immunoprecipitation (10-20 micro;g/ml). However, the optimal conditions should be determined individually. The phospho-Akt (Ser473) antibody detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bAkt, also referred to as PKB and RAC, is the cellular homologue of the viral oncogene v-Akt. This protein kinase is activated by insulin and various growth factors and functions in a pathway involving PI3 kinase as activation is sensitive to wortmanin. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis, although the pathways involved have not been identified. Akt is activated by phospholipid binding and phosphorylation within the activation loop at Thr308 and within the C-terminus at Ser473./p