Phosphatidylcholine Colorimetric/Fluorometric Assay Kit
Kits
- 貨號 Cat. No.
- K576-100
- 規格 Size
- K576-100
- Sample Type
- as well as many other biological fluids 、 Cell and tissue culture supernatants 、 Plasma 、 Serum 、 urine
Description
Alternate Name :
Tag Line :
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Species reactivity- Mammalian
• Applications- BioVision’s Phosphatidylcholine kit measures PC in the range of 0.1 to 10 nmol per sample.
Detection Method:
Features & Benefits:
• Simple procedure; takes less than ~40 minutes
• Fast and convenient
Description:
Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid. BioVision's Phosphatidylcholine Assay Kit is a simple convenient means of measuring Phosphatidylcholine in a variety of biological samples. The kit utilizes an enzyme-coupled assay in which PC is hydrolyzed, releasing choline which is subsequently oxidized resulting in development of the OxiRed probe to generate fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). BioVision’s Phosphatidylcholine kit measures PC in the range of 0.1 to 10 nmol per sample. PC is present in serum at ~ 0.2-2.5 mM (~50-200 mg/dL).