Phenylalanine Fluorometric Assay Kit
Kits
- 貨號 Cat. No.
- K572-100
- 規格 Size
- K572-100
- Sample Type
- as well as many other biological fluids 、 Cell and tissue culture supernatants 、 Plasma 、 Serum 、 urine
Description
Alternate Name :
Tag Line :
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Fluorescence (Ex/Em 535/587 nm)
• Species reactivity- Mammalian
• Applications- The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.
Detection Method:
Features & Benefits:
• Simple procedure; takes less than ~30 minutes
• Fast and convenient
Description:
L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences. BioVision’s Phenylalanine Assay Kit provides a quick, simple, accurate method for quantifying PHE in biological samples. In the assay, PHE is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.