Phenylalanine Assay Kit (Colorimetric)

Kits

貨號 Cat. No.
K481-100
規格 Size
K481-100
Reactivity
Eukaryotes
Sample Type
as well as many other biological samples 、 Cell and tissue culture supernatants 、 Plasma 、 Serum 、 urine

Description




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Storage Conditions: -20°C


 

Shelf life: 12 months


 

Summary :


• Detection method- Absorbance (OD: 450 nm)
• Species reactivity- Mammalian
• Applications- The assay is linear in the range from 1.0 to 10 nmol (1-50 µM) of Phenylalanine.


 

Detection Method: Absorbance (450 nm)


 

Features & Benefits:


• Simple procedure; takes less than ~60 minutes
• Fast and convenient


 

Description:


L-Phenylalanine (Phe) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids building the polypeptide backbone. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in Phe metabolism lead to phenylketonuria or PKU which can have dire consequences. BioVision’s Phenylalanine Assay Kit (Colorimetric) provides a quick, simple, accurate method for quantifying Phe in biological samples. In the assay, Phe is metabolized with the simultaneous formation of NADH which reacts with a probe to generate absorbance that can be followed colorimetrically at 450 nm. The assay is linear in the range from 0.2 to 10 nmol, allowing accurate determination of concentrations in the range of 1 - 50 µM Phenylalanine.


 





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