PEDF Antibody (LS-B2471)

貨號 Cat. No.: LS-B2471
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2471
Antibody Validation:	This antibody replaces catalog number LS-C17230. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Pigment Epithelium-derived Factor (SERPINF1)
Synonyms:	SERPINF1, EPC-1, PEDF, PIG35, pigment epithelium-derived factor, Serpin-F1
Host:	SERPINF1 antibody was produced in Mouse
Antigen Species:	SERPINF1 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG2a
Antigen Type:	Recombinant protein
Immunogen:	SERPINF1 antibody was raised against recombinant human GST-PEDF fusion protein. The sequence corresponding to aa196-347 of human PEDF tagged with GST on the N-terminal was expressed in E. coli. GST-PEDF is about 46kD on SDS-PAGE.
Antibody Specificity:	Recognizes human GST-PEDF fusion protein with a MW of ~46kD.
Antigen Modification:	aa196-347
Purification:	Protein A Column
Presentation:	PBS, pH 7.6, 0.1% sodium azide.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2471 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2471 was determined to be 5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunohistochemistry - Frozen (IHC-Fr), Immunocytochemistry (ICC), Western blot (WB), ELISA (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	1 mg/ml