Proteins and Peptides
PDK1 Antibody (LS-B1733)
- 貨號 Cat. No.
- LS-B1733
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B1733 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C2831. It has been validated for use in the following assays: IHC. |
| Antibody Target: | Pyruvate Dehydrogenase Kinase Isoenzyme 1 (PDK1) |
| Synonyms: | PDK1, PDH kinase 1, PDHK, PDHK1, pdk1, Pyruvate dehydrogenase kinase 1, pyruvate dehydrogenase kinase isoenzyme 1 |
| Family: | Protein Kinase |
| Subfamily: | PDK/BCKDK |
| Host: | PDK1 antibody was produced in Rabbit |
| Antigen Species: | PDK1 antibody was raised against Human |
| Clonality: | Polyclonal |
| Antigen Type: | Synthetic peptide - KLH conjugated |
| Immunogen: | PDK1 antibody was raised against synthetic peptide |
| Antibody Specificity: | A synthetic peptide made to a C-Terminal portion of the human PDK1 protein sequence (between residues 350-436). [Swiss-Prot Q15118]. |
| Antigen Modification: | aa350-436 |
| Predicted Species Reactivity: | Mouse, Rat |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | Tris-glycine buffer, 150 mM sodium chloride. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B1733 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1733 was determined to be 5 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (2 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 0.4 mg/ml |


