PDCD1 Antibody (LS-B2519)

貨號 Cat. No.
LS-B2519
規格 Size
50 µg
品牌 Brand
LSBio

Description

























































































Antibody Catalog ID: LS-B2519
Antibody Validation: This antibody replaces catalog number LS-C60134. It has been validated for use in the following assays: IHC-P.
Antibody Target: Programmed Cell Death 1 (PDCD1)
Synonyms: PDCD1, CD279, HPD-1, HPD-l, PD1, SLEB2
Family: Apoptosis
Subfamily: Not assigned
Host: PDCD1 antibody was produced in Rabbit
Antigen Species: PDCD1 antibody was raised against Human
Clonality: Polyclonal
Antigen Type: Synthetic peptide
Immunogen: PDCD1 antibody was raised against synthetic peptide corresponding to a region near the carboxy terminus of human PDCD1 protein
Antibody Specificity: human PDCD1 protein. This antibody reacts with PDCD1 from human, mouse and rat sources. Reactivity with PDCD1 from other sources has not been determined
Antigen Modification: C-Terminus
Predicted Species Reactivity: Mouse, Rat
Purification: Immunoaffinity Chromatography
Presentation: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.02% sodium azide.
Recommended Storage: +4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary: Immunohistochemistry: LS-B2519 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2519 was determined to be 2.5 ug/ml. Western Blot: Expect a band at ~47 kD in size corresponding to PDCD1 in the appropriate cell lysate or extract. THP-1 cell lysate can be used as positive control.
Antibody Uses: Immunohistochemistry - Paraffin (IHC-P) (2.5 µg/ml), Western blot (WB) (0.5 - 1 µg/ml), ELISA (1:10000 - 1:40000) (Optimal dilution to be determined by the researcher)
Size: 50 µg
Concentration: 1 mg/ml

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