Proteins and Peptides
PDCD1 Antibody (LS-B2519)
- 貨號 Cat. No.
- LS-B2519
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2519 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C60134. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Programmed Cell Death 1 (PDCD1) |
| Synonyms: | PDCD1, CD279, HPD-1, HPD-l, PD1, SLEB2 |
| Family: | Apoptosis |
| Subfamily: | Not assigned |
| Host: | PDCD1 antibody was produced in Rabbit |
| Antigen Species: | PDCD1 antibody was raised against Human |
| Clonality: | Polyclonal |
| Antigen Type: | Synthetic peptide |
| Immunogen: | PDCD1 antibody was raised against synthetic peptide corresponding to a region near the carboxy terminus of human PDCD1 protein |
| Antibody Specificity: | human PDCD1 protein. This antibody reacts with PDCD1 from human, mouse and rat sources. Reactivity with PDCD1 from other sources has not been determined |
| Antigen Modification: | C-Terminus |
| Predicted Species Reactivity: | Mouse, Rat |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.02% sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2519 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2519 was determined to be 2.5 ug/ml. Western Blot: Expect a band at ~47 kD in size corresponding to PDCD1 in the appropriate cell lysate or extract. THP-1 cell lysate can be used as positive control. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (2.5 µg/ml), Western blot (WB) (0.5 - 1 µg/ml), ELISA (1:10000 - 1:40000) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |


