PD-L1 Protein, Human, Recombinant (His Tag)

PD-L1 Protein, Human, Recombinant (His Tag): Product Information

Purity

> 98 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

1. Measured by its binding ability in a functional ELISA. Immobilized human B7-H1 at 20 μg/ml (100 μl/well) can bind human PD1 with a linear ranger of 0.032-0.8 μg/ml.

2. Using the Octet RED System, the affinity constant (Kd) of PD-1 Protein, Human, Recombinant (Fc Tag) (Cat. 10377-H02H) bound PD-L1 Protein, Human, Recombinant (His Tag) (Cat. 10084-H08H) was 19.2nM.

Protein Construction

A DNA sequence encoding the N-terminal segment (Met 1-Thr 239) of the extracellular domain of human B7-H1 (NP_054862.1) was expressed with a C-terminal polyhistidine tag.

Accession#

NP_054862.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Phe 19

Molecule Mass

The recombinant mature human B7-H1 comprises 232 amino acids and predicts a molecular mass of 26.8 kDa. As a result of glycosylation, the rh B7-H1 migrates as an approximately 35-38 kDa protein in SDS-PAGE under reducing conditions.