PD 98059
- 貨號 Cat. No.
- P-260
- 規格 Size
- 10 x 0.1 mg 1mg 5x1mg
Description
PD 98059
Potent Inhibitor of Mitogen-Activated Protein Kinase (MAPK) Pathway
| Product#: | P-260 |
| Sizes: |
| 10 x 0.1 mg |
| 1 mg |
| 5 x 1 mg |
is a synthetic flavonoid and a potent inhibitor of mitogen-activated protein kinase (MAPK) by inhibiting its immediate upstream activator, mitogen activated protein kinase kinase (also known as MEK or MKK1).1-3
PD 98059 arrests cell cycle progression of some rat cultured cells at the G0/G1 phase and blocks nerve growth factor stimulated axonal outgrowth from adult mouse dorsal root ganglia in vitro,4-5 and blocks induction of LTP in the brain areas.6-7
PD 98059 is also an equipotent antagonist of aryl hydrocarbon receptor (AHR) stimulated by TCDD in MCF10A cells, 8-10
| M.W.: | 360.21 |
| CAS No.: | [167869-21-8] |
| Structure: |
 |
| Purity: | >99% |
| Bioassay: |
| PD 98059 inhibits phophorylation of P42/44 MAPK (ERK) stimulated by PDGF. |
 |
C6 glioma cells were grown to 70% confluence and then serum starved for 1.5 h. The cells were incubated for 2 h with various concentrations of PD 98059 as indicated in the picture and stimulated with 7ng/ml PDGF-AA. The cell proteins were resolved by SDS PAGE and probed with anti-phospho-P42/44 MAPK (upper panel), stripped and followed by reprobing with anti-P42/44 MAPK (lower panel). The inhibition of P42/44 MAPK phosphorylation by PD 98059 is dose dependent. |
| References: |
1. | Alessi, D.R., et al. (1995) J. Biol. Chem. 270, 27489. |
2. | Dudley, D.T., et al. (1995) Proc. Natl. Acad. Sci. 92, 7686. |
3. | Pang, L. et al. (1995) J. Biol. Chem. 270, 13585. |
4. | Yamaguchi, K. et al. (2002) J. Ophthalmol. 46, 634. |
5. | Sjogreen, B. et al. (2000) Neuroscience 100, 407. |
6. | Andrew, N. et al. (1999) J. Neurophysiol. 81, 103. |
7. | English, J. D. et al. (1997) J. Biol. Chem. 272, 19103. |
8. | Guyton, K.Z. et al. (1996) Cancer Res. 36, 3480. |
9. | Cook, S.J. et al. (1997) J. Biol. Chem. 272,13309. |
10. | John, J. et al. (1998) Mol. Pharmacol. 53, 438. |
PD 98059