PC-12
- 貨號 Cat. No.
- CLC030
- 品牌 Brand
- gene-rl
Description
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Cell Lines (GRL-CLC030) | |
Designations | PC-12 |
| Cell type | Rat pheochromocytoma adrenal |
Growth property | Suspension |
Seeding density(cells/ml) | 2.0x106 |
Survival rate | 64% |
Culture medium | 82.5%Ham’s F12K with 2mM L-glutamine+15% horse serum+2.5% FBS |
| Incubation condition | 370C 5% CO2 |
Freezed medium | 90% culture medium+10%DMSO |
Medium renewal | every 2 to 3 days |
| Subculturing |
Remove cells from substrate by pipetting. To obtain single cells pass the cell suspension through a syringe with a 22 gauge needle several times. The PC-12 cells adhere poorly to plastic and tend to grow in small clusters. Subcultivation ratio: A subcultivation ratio of 1:3~1:8 is recommended |
Mycoplasma test | Negative for bacteria，fungi and mycoplasma. |
Advertence: |
When you receive the cell line, please examine the flask and its cap. If it is not well-found, please tell us instantly. After examination, please wipe the flask and flask cap with 70% alcohol, and then loosen it. Afterward, put the flask into incubator immediately.