PAX6 Polyclonal Antibody

FORMULATION: 100 ug (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.

SPECIES REACTIVITY: human, mouse, rat, sheep, chicken, frog

STORAGE CONDITIONS: Store at -20oC. For long-term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles.

BACKGROUND DESCRIPTION: PAX genes encode nuclear transcription factors that may function as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters' anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.

SPECIFICITY: The antibody detects 47 kDa PAX6 protein in samples from human, mouse, rat, sheep, chicken, and frog origins.

APPLICATION AND USAGE: The antibody performs well in Western blot analysis (1-4 ug/ml). However, the optimal conditions should be determined individually. Other applications have not been tested.

NOTE: For Research Use Only! Not to be used in human.