Proteins and Peptides
PAX2 Antibody (LS-B2450)
- 貨號 Cat. No.
- LS-B2450
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2450 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C9503. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | PAX2 |
| Synonyms: | PAX2 Antibody, paired box protein pax-2 Antibody |
| Host: | PAX2 antibody was produced in Rabbit |
| Antigen Species: | PAX2 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide |
| Immunogen: | PAX2 antibody was raised against synthetic phospho-peptide derived from the sequence surrounding the phosphorylated Ser393 residue of mouse and human Pax2 (paired box protein 2). |
| Antibody Specificity: | Recognizes human Pax2 protein when phosphorylated at Ser393. Based on amino acid sequence homology with Pax5 and Pax8, cross-reactivity with these proteins is likely. Does not react with purified, unphosphorylated GST-Pax2 proteins. Reacts with human HEK293 cell lysates and purified GST-Pax2 proteins phosphorylated by JNK. Species sequence Homology: mouse, chicken, zebra sh and frog - 100%. |
| Antigen Modification: | pSer393 |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | PBS, pH 7.4, 0.1% sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2450 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2450 was determined to be 1:50. Western Blot: Analysis of JNK-stimulated (phosphorylated) fusion proteins identifies a single band at ~50kD. In Western Blot analysis of HEK293 cells, a thick band between ~42-45kD is observed, which may represent different human Pax2 isoforms and/or Pax8. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1:50), Western blot (WB) (1 - 3 µg/ml), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.25 mg/ml |
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