PARP Antibody

Antibody Target :PARP

Host: Mouse

Antibody Type: Monoclonal

Isotype: Mouse IgG1

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Purified PARP from Calf thymus

Accession#: N/A

Gene ID: N/A

Appearance: Colorless liquid

Concentration: 0.2 mg/ml

Formulation: 100 µg (0.2 mg/ml) protein A purified IgG1 in PBS containing 0.5% BSA and 0.05% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms/rt/ht/mk

Application: WB/IC

Application & Usage: Western Blotting (1-4 µg/ml) and immunocytochemistry (10-20 ug/ml). Recognize 116 kDa PARP and the 85 kDa apoptosis-related cleavage fragment. However, the optimal conditions should be determined individually.

Storage Temp.: -20°C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: PARP is a highly conserved nuclear enzyme present in higher eukaryotes. The enzyme is a Zn2+-dependent DNA binding protein that recognizes DNA strand breaks and is implicated in DNA repair and in apoptosis responses of cells. As a marker for apoptosis, it has been shown that PARP cleavage mediated by caspase-3 activity occurs at the onset of apoptosis, and inhibition of PARP cleavage attenuates apoptosis in vitro.