PAR4 Antibody

Antibody Target :PAR4

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic peptide surrounding amino acid 327 of human Par4

Accession#: EAW97348

Gene ID: 5074

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms/rt/dg/hs

Application: WB/IP/IHC

Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes PAR4 in samples from of human, mouse, and rat origins. Reactivity to other species has not been tested.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: Five ionomycin-inducible complementary cDNAs, designated PAR1, 2, 3, 4 and 5, have been isolated from the prostate cancer cell line AT-3. Nucleotide sequencing identified PAR1 as the rat homolog of MKP-1, PAR2 as the injury-inducible gene HB-EGF, and PAR3 as the serum-induced gene cyr-61. PAR4 and PAR5 sequences were not found to correspond to any previously described proteins. PAR4 (prostate apoptosis response-4) is specifically expressed by cells entering apoptosis and is not inducible by growth factor stimulation, oxidative stress and necrosis, or growth arrest. The PAR4 gene encodes a 38 kDa protein with a putative nuclear localization signal and carboxy terminal leucine zipper.