PAK1/2 (P429) pAb

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h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
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h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
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h3 class=list6_t1Specificity/h3
p class=list6_textPAK1/2 (P429) antibody detects endogenous levels of PAK1/2 protein./p
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h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textWB: 1:500~1:1000 IHC: 1:50~1:200/p
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h3Western Blot(WB)/h3
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div style=width: 250pxWestern blot (WB)analyzes of PAK1/2 (P429) antibody in extracts from CV-1 cells./div
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h3Immunohistochemistry (IHC)/h3
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pImmunohistochemistry (IHC) analyzes of PAK1/2 (P429) antibody in paraffin-embedded human brain tissue./p
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h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4deg;C short term. Aliquot and store at -20deg;C long term. Avoid freeze-thaw cycles./p
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h3 class=list6_t1Background/h3
p class=list6_textThree isoforms of serine/threonine kinases, designated alpha;PAK p68, Beta;pak p65 and gamma;PAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha;, beta; and gamma;PAK isoforms complex specifically with Rac 1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gamma;PAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gamma;PAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway./p
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