PAI-1 mutant - stable, no LRP binding

PAI-1 mutant - stable, no LRP binding

A substitution of Glutamic Acid for Arginine at position 76 greatly decreases the binding of this Human PAI-1 mutant to the low density lipoprotein receptor-related protein (LRP). The putative LRP binding exosite is thought to overlap with the heparin binding site on the PAI-1 molecule. Binding to LRP or similar receptors leads to the clearance of PAI-1 complexes. An additional mutation provides increased stability for use in long term binding experiments or in vivo studies.