p70 S6 Kinase Antibody

Antibody Target :p70 S6 Kinase

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic peptide near the C-terminus of human p70 S6 kinase

Accession#: Q9UBS0

Gene ID: 6199

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) antigen affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.

Purification: Affinity purified

Species Reactivity: hu/ms/rt

Application: WB/IP/IHC

Application & Usage: Western blotting (1 µg/ml), immunoprecipitation (10-20 µg/ml), and immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes p70 S6 kinase of human, mouse, and rat origins. Reactivity to other species has not been tested.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: p70 S6 kinase and p90 Rsk kinase are the two distinct 40S ribosomal protein S6 Ser/Thr kinases present in somatic animal cells. p90 Rsk kinase is maximally activated within minutes of addition of growth factors or phorbol ester to cultured cells followed by activation of p70 S6 kinase. Both enzymes are regulated by serine/threonine phosphorylation, suggesting that specific kinases may exist upstream in the signaling pathways that regulate these kinases.