Proteins and Peptides
P4HB Antibody (LS-B2372)
- 貨號 Cat. No.
- LS-B2372
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2372 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C38114. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Prolyl 4-hydroxylase, Beta Subunit (P4HB) |
| Synonyms: | P4HB, DSI, ERBA2L, GIT, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, protein disulfide isomerase |
| Host: | P4HB antibody was produced in Rabbit |
| Antigen Species: | P4HB antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide |
| Immunogen: | P4HB antibody was raised against synthetic peptide derived from the sequence around Pro329 of human PDI (KLH). |
| Antibody Specificity: | Recognizes endogenous levels of total human PDI protein. Species cross-reactivity: monkey, mouse and rat. |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | Supplied as a liquid in 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2372 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2372 was determined to be 1:50. Immunofluorescence (IF-IC): 1:25. Requires methanol permeabilization. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1:50), Immunocytochemistry (ICC), Western blot (WB), Immunofluorescence (IF) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
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