p-Sp1 (T739) pAb
- 貨號 Cat. No.
- BS4755
- 規格 Size
- 100 μg
- 品牌 Brand
- Bioworld
Description
div class=list6_content
div class=list6_li
h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
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div class=list6_li
h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
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h3 class=list6_t1Specificity/h3
p class=list6_textp-Sp1 (T739) antibody detects endogenous levels of p-Sp1 protein./p
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h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textWB: 1:500~1:1000/p
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div id=westernpic
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h3Western Blot(WB)/h3
img id=wpic src=http://www.bioworlde.com/upload/web/4755.jpg width=120 alt= /
div style=width: 250pxWestern blot (WB) analyzes of p-Sp1 (T739) antibody in extracts from COS serum cells./div
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h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4/p
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div class=list6_li
h3 class=list6_t1Background/h3
p class=list6_textSp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21 bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A), may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.1./p
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div class=list6_li
h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
/div
div class=list6_li
h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
/div
div class=list6_li
h3 class=list6_t1Specificity/h3
p class=list6_textp-Sp1 (T739) antibody detects endogenous levels of p-Sp1 protein./p
/div
div class=list6_li
h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textWB: 1:500~1:1000/p
/div
div id=pic
div id=westernpic
div class=imagecontainer
h3Western Blot(WB)/h3
img id=wpic src=http://www.bioworlde.com/upload/web/4755.jpg width=120 alt= /
div style=width: 250pxWestern blot (WB) analyzes of p-Sp1 (T739) antibody in extracts from COS serum cells./div
/div
/div
/div
div class=list6_li
h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4/p
/div
div class=list6_li
h3 class=list6_t1Background/h3
p class=list6_textSp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21 bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A), may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.1./p
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