p-PKD1/PKC-mu (S910) pAb

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h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
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h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
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h3 class=list6_t1Specificity/h3
p class=list6_textp-PKD1/PKC-mu (S910) antibody detects endogenous levels of p-PKD1/PKC-mu protein./p
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h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textWB: 1:500~1:1000 IHC: 1:50~1:200/p
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h3Western Blot(WB)/h3
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div style=width: 250pxWestern blot (WB) analysis of p-PKD1/PKC-mu (S910) antibody in extracts from NIH/3T3 PDGF cells ./div
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h3Immunohistochemistry (IHC)/h3
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pImmunohistochemistry (IHC) analyzes of p-PKD1/PKC-mu (S910) antibody in paraffin-embedded human brain tissue./p
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h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4deg;C short term. Aliquot and store at -20deg;C long term. Avoid freeze-thaw cycles./p
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h3 class=list6_t1Background/h3
p class=list6_textMembers of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha;, beta;I, beta;II and gamma;) and novel (n) PKC isoforms (delta;, epsilon; , zeta;, eta; and theta;). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta; and epsilon; are independent of Ca++. On the other hand, nPKC delta; and epsilon;, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities./p
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