p-PI3K p85 (Y458)/p55 (Y199) Antibody

pa href=http://www.bioworlde.com/ACE2-%28K465%29-Antibodyimg alt= src=http://www.bioworlde.com/best/template/product/image/img11.gif //astock/p
pimg alt= src=file:///C:/Users/jimmy/AppData/Local/Temp/moz-screenshot-2.jpg //p
h3 class=list6_t1Host: Rabbit/h3
h3 class=list6_t1Reactivity: Human,Mouse,Rat/h3
p class=list6_t1nbsp;/p
div class=list6_li
h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
/div
div class=list6_li
h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
/div
div class=list6_li
h3 class=list6_t1Specificity/h3
p class=list6_textAmyloid beta; A4 (E737) antibody detects endogenous levels of Amyloid beta; A4 protein./p
/div
div class=list6_li
h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textWB: : 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200/p
/div
div id=pic
div id=westernpic
div class=imagecontainer
h3Western Blot(WB)/h3
img id=wpic src=http://www.bioworlde.com/upload/web/4605.jpg width=120 alt= /
div style=width: 250pxWestern blot (WB) analysis of p-PI3K p85 (Y458)/p55 (Y199) antibody in extracts from NIH/3T3 cells./div
/div
/div
div id=IHCpic
div class=imagecontainer
h3Immunohistochemistry (IHC)/h3
img id=ipic height=180 src=http://www.bioworlde.com/upload/ihc/4605.jpg width=240 alt= //div
pImmunohistochemistry (IHC) analyzes of p-PI3K p85 (Y458)/p55 (Y199) antibody in paraffin-embedded human breast carcinoma tissue./p
/div
/div
div class=list6_li
h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4deg;C short term. Aliquot and store at -20deg;C long term. Avoid freeze-thaw cycles./p
/div
div class=list6_li
h3 class=list6_t1Background/h3
p class=list6_textThe enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues./p
/div