p-PDGFR beta (Y751) pAb

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h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
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h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
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h3 class=list6_t1Specificity/h3
p class=list6_textp-PDGFR beta (Y751) antibody detects endogenous levels of p-PDGFR beta protein./p
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h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textWB: 1:500~1:1000 IHC: 1:50~1:200/p
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h3Western Blot(WB)/h3
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div style=width: 250pxWestern blot (WB) analysis of p-PDGFR beta (Y751) antibody in extracts from NIH/3T3 PDGF cells ./div
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h3Immunohistochemistry (IHC)/h3
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pImmunohistochemistry (IHC) analyzes of p-PDGFR beta (Y751) antibody in paraffin-embedded human brain tissue./p
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h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4deg;C short term. Aliquot and store at -20deg;C long term. Avoid freeze-thaw cycles./p
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h3 class=list6_t1Background/h3
p class=list6_textPlatelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and gliaderived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-alpha; and -beta;, which are endowed with protein tyrosine kinase domains. PDGFR-alpha; can bind to both A and B subunits of PDGF, while PDGFR-beta; can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two alpha; receptors, PDGF-AB induces dimerization of alpha;alpha; and alpha;beta;, and PDGF-BB induces the formation of three types of dimers, alpha;alpha;, alpha;beta; and beta;beta;. Translocation of the PDGFR-beta; gene with the TEL gene is linked with chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors./p
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