p-PAK1/2 (T423/402) pAb
- 貨號 Cat. No.
- BS5029
- 規格 Size
- 100 μg
- 品牌 Brand
- Bioworld
Description
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h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
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h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
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h3 class=list6_t1Specificity/h3
p class=list6_textp-PAK1/2 (T423/402) antibody detects endogenous levels of p-PAK1/2 protein./p
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h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textIHC: 1:50~1:200/p
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div id=IHCpic
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h3Immunohistochemistry (IHC)/h3
img id=ipic src=http://www.bioworlde.com/upload/ihc/5029.jpg width=240 height=180 alt= //div
pImmunohistochemistry (IHC) analyzes of p-PAK1/2 (T423/402) antibody in paraffin-embedded human brain tissue./p
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h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4deg;C short term. Aliquot and store at -20deg;C long term. Avoid freeze-thaw cycles./p
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h3 class=list6_t1Background/h3
p class=list6_textThree isoforms of Serine/Threonine kinases, designated alpha;PAK p68, beta;PAK p65 and gamma;PAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha;, beta; and gamma;PAK isoforms complex specifically with Rac 1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. The site of autophosphorylation on alpha;PAK is Thr 423, which is in the kinase activation loop. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK-4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac 1 and Cdc42, they do not interact with Rho./p
/div
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div class=list6_li
h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
/div
div class=list6_li
h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
/div
div class=list6_li
h3 class=list6_t1Specificity/h3
p class=list6_textp-PAK1/2 (T423/402) antibody detects endogenous levels of p-PAK1/2 protein./p
/div
div class=list6_li
h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textIHC: 1:50~1:200/p
/div
div id=pic
div id=IHCpic
div class=imagecontainer
h3Immunohistochemistry (IHC)/h3
img id=ipic src=http://www.bioworlde.com/upload/ihc/5029.jpg width=240 height=180 alt= //div
pImmunohistochemistry (IHC) analyzes of p-PAK1/2 (T423/402) antibody in paraffin-embedded human brain tissue./p
/div
/div
div class=list6_li
h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4deg;C short term. Aliquot and store at -20deg;C long term. Avoid freeze-thaw cycles./p
/div
div class=list6_li
h3 class=list6_t1Background/h3
p class=list6_textThree isoforms of Serine/Threonine kinases, designated alpha;PAK p68, beta;PAK p65 and gamma;PAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha;, beta; and gamma;PAK isoforms complex specifically with Rac 1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. The site of autophosphorylation on alpha;PAK is Thr 423, which is in the kinase activation loop. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK-4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac 1 and Cdc42, they do not interact with Rho./p
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