p-Histone H3.1 (S28) pAb
- 貨號 Cat. No.
- BS5023
- 規格 Size
- 100 μg
- 品牌 Brand
- Bioworld
Description
Product
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
p-Histone H3.1 (S28) antibody detects endogenous levels of p-Histone H3.1 protein.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IHC: 1:50~1:200
Western Blot(WB)
Western blot (WB) analysis of p-Histone H3.1 (S28) antibody in extracts from HuvEc Serum cells .
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of p-Histone H3.1 (S28) antibody in paraffin-embedded human malignant lymphoma tissue.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of Histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H3, the core protein of the nucleosome, becomes phosphorylated at the end of prophase. The two major sites of phosphorylation are the mitosis-specific site Ser 10 and Ser 28, both of which are extensively phosphorylated in DNA-bound forms of Histone H3 and in nucleosomal Histone H3. The nucleosome structure of Histone H3 promotes N-terminal phosphorylation in vitro. Phosphorylation of Histone H3 at Ser 10 is an essenti
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